Table of Contents

Preface, L.M. Schwartz and B.A. Osborne; Anatomical Methods in Cell Death, J.F.R. Kerr, G.C. Gobe, C.M. Winterford, and B.V. Harmon; Identification of Dying Cells-In Situ Staining, S.A. Ben-Sasson and Y. Gavrieli; Assays of DNA Fragmentation, Endonucleases, and Intracellular pH and Ca2+ Associated with Apoptosis, Quantitation of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer, A. Eastman; P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford; Cell Cycle Analysis of Apoptosis Using Flow Cytometry, S. W. Sherwood and R. T Schimke; Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes, B.A. Osborne, S. Smith, Z.-G. Liu, K. McLaughlin, and L.M. Schwartz; Cloning Cell Death Genes, L.M. Schzwartz, C.E. Milligan, W. Bielke, and S.J. Robinson; Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death, L.T. Bemis, F.J. Beske, and R. Strange; The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro, A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J.. Mogil, and D.R. Green; Calcium, Free Radicals, and Excitotoxic Neuronal Death in Primary Cell Culture, M. P. Mattson, S. W. Barger, J. G. Begley, and R.I. Mark; Use of Cultured Neurons and Neuronal Cell Lines to Study Morphological, Biochemical, and Molecular Changes Occurring in Cell Death, J.C. Mills, S. Wang, M. Ericinska, and R.N. Pittman; Methods for Studying Cell Death and Viability of Primary Neuronal Cultures, J.E. Johnson; Neuron Death in Vertebrate Development: In Vivo Methods, P.G.H. Clarke and R. W. Oppenheim; Methods for the Study of Cell Death in the Nematode Caenorhabditis elegans, M. Driscoll; Programmed Cell Death during Mammary Gland Involution, R. Strange, R.R. Frits, L.T. Bemis, and F.J. Geske; Methods for the Study of Apoptosis in the Ventral Prostate Gland, R. Buttyan; Genetic Approaches for Studying Programmed Cell Death during Development of the Laboratory Mouse, E.C. Coucouvanis, G.R. Martin, and J.H. Nadeau.

About the Author

Edited by Leslie I. Wilson, Paul T. Matsudaira, Lawrence M. Schwartz, and Barbara A. Osborne