Table of Contents

Construction and Evaluation of Zinc Finger Nucleases.- Updated overview of TALEN construction systems.- CRISPR/Cas9.- SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System.- Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design.- Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA.- Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing.- Generation of Knock-in Mouse by Genome Editing.- Introduction of genetic mutations into mice by Base Editor and Target-AID.- Genome editing in mouse and rat by electroporation.- Generation of floxed mice by sequential electroporation.- Efficient detection of flox mice using in vitro Cre recombination.- VCre/VloxP and SCre/SloxP as reliable site-specific recombination systems for genome engineering.- Efficient CRISPR/Cas9-Assisted Knockin ofLarge DNA Donors by Pronuclear Microinjection During S-phase in Mouse Zygotes.- Genome editing of murine liver hepatocytes by AAV vector-mediated expression of Cas9 in vivo.- Non-Viral Ex Vivo Genome-Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9.- Genome editing of rat.- A simple and efficient method for generating KO rats using in vitro fertilized oocytes.- Editing the Genome of the Golden Hamster (Mesocricetus auratus).- Gene targeting in rabbits: single-step generation of knockout rabbits by microinjection of CRISPR/Cas9 plasmids.- Genome Editing of Pig.- GEEP method: an optimized electroporation-mediated gene-editing approach for establishment of knockout pig lines.- Genome editing mediated by primordial germ cell in chicken.- CRISPR–Cas9-mediated genome modifications in zebrafish.- Genome Editing of Medaka. -CRISPR-Cas9 based functional analysis in amphibians; Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl.- Genome Editing of Silkworms.- Improved genome editing in the ascidian Ciona with CRISPR/Cas9 and TALEN.- Genome editing of C. elegans.