Table of Contents

Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling.- Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells.- Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Micro copy Images of C. elegans Embryos.- Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells.- Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics.- The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton.- Measuring Microtubule Growth and Gliding in C. elegans Embryos.- Xenopus Egg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators.- Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays.- An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules.- Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins.- The Segmentation of Microtubules in Electron Tomograms using Amira.- Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers.- Four color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells.