Table of Contents

Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b; Biochemical Analyses of the Wrch Atypical Rho Family GTPases; Purification of P-Rex1 from neutrophils and nucleotide exchange assay; In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/ CZH2 domains; Biochemical characterization of the Cool (Coned-out-of-Library)/Pix (Pak-interactive exchange factor) proteins; GEF and glucosylation assays on liposome bound Rac; Phosphorylation of RhoGDI by p21-activated kinase 1; Purification of ARAP3 and characterization of GAP activities; Regulation of RhoGAP specificity by phospholipids and prenylation; Purification and activity of the Rho ADP-ribosylating binary C2/C3 toxin; Purification of Tat-C3 exoenzymes; Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation ; Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts; In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components; Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics; Formin proteins: purification and measurement of effects on actin assembly; Purification and Kinase Assay of PKN; Purification and Enzyme Activity of ACK1; Direct activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid vesicles; Regulation of PLCâ isoforms by Rac; Biochemical properties and inhibitors of (N-)WASP; The Use of GFP to localize Rho GTPases in Living Cells; Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes; Measurement of Activity of Rho GTPases during Mitosis; RNAi inhibition of Rho GTPases; RNA interference techniques to study epithelial cell adhesion and polarity; Nucleofection of primary neurons; Dock180-ELMO Cooperation in Rac Activation; Rho GTPase activation by cell-cell adhesion; Activation of Rap1, Cdc42, and Rac by nectin adhesion system; Analysis of activated GAPs and GEFs in cell lysates; Degradation of RhoA by Smurf1 ubiquitin ligase; Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin; Regulation of superoxide-producing NADPH in non-phagocytic cells; Activation of MEKK1 by Rho GTPases; Activation of Apoptotic JNK Pathway through the Rac1-Binding Scaffold Protein POSH; Quantification of isozyme-specific activation of phospholipase C-â2 by Rac GTPases and phospholipase C-å by Rho GTPases in an intact cell assay system; Activation of Rho and Rac by Wnt/Frizzled signalling; Fluorescent assay of cell permeable C3 transferase activity; Use of TIRF microscopy to visualize actin and microtubules in migrating cells; Inhibition of ROCK by RhoE; Conditional regulation of a ROCK-estrogen receptor fusion protein; Rational design and applications of a Rac GTPase-specific small molecule inhibitor; In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization; Real time centrosome reorientation during fibroblast migration; Lentiviral delivery of RNAi in Hippocampal Neurons; Methods for studying neutrophil Chemtoaxis; Measurement of Epidermal Growth Factor Receptor turnover and effects of Cdc42; Tumor cell migration in three dimensions; Reciprocal regulation of cyclin D1 expression by Rac and Rho; Regulation of ERK signal duration and cyclin D1 expression by Rhodependent stress fiber formation; An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration; Analysis of a Mitotic role of Cdc42; Plexin-induced collapse assay in COS cells; Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures; Using Three Dimensional Acinar Structures for Molecular and Cell Biological Assays; TC10 and insulin-stimulated glucose transport; GTPases and the control of neuronal polarity; In vitro assembly of filopodia-like bundles

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